| Player Name | Jersey No | Pos | HT | WT | Age | Exp | College |
| Alex Anzalone | 34 | LB | 6-3 | 237 | 27 | 6 | Florida |
| Derrick Barnes | 55 | LB | 6-0 | 238 | 23 | 2 | Purdue |
| David Blough | 10 | QB | 6-1 | 207 | 27 | 4 | Purdue |
| Chris Board | 49 | LB | 6-2 | 239 | 27 | 5 | North Dakota State |
| Michael Brockers | 90 | DL | 6-5 | 297 | 31 | 11 | LSU |
| Evan Brown | 63 | C | 6-3 | 320 | 25 | 4 | Southern Methodist |
| Austin Bryant | 2 | DL | 6-5 | 250 | 25 | 4 | Clemson |
| Isaiah Buggs | 98 | DL | 6-3 | 295 | 26 | 4 | Alabama |
| Quintez Cephus | 87 | WR | 6-1 | 208 | 24 | 3 | Wisconsin |
| DJ Chark | 4 | WR | 6-4 | 205 | 25 | 5 | LSU |
| John Cominsky | 79 | DL | 6-5 | 285 | 26 | 4 | Charleston |
| Scott Daly | 47 | LS | 6-1 | 245 | 28 | 2 | Notre Dame |
| Taylor Decker | 68 | T | 6-7 | 320 | 29 | 7 | Ohio State |
| DeShon Elliott | 5 | S | 6-1 | 210 | 25 | 5 | Texas |
| Jack Fox | 3 | P | 6-2 | 215 | 26 | 3 | Rice |
| Jared Goff | 16 | QB | 6-4 | 217 | 27 | 7 | California |
| Charles Harris | 53 | DL | 6-3 | 250 | 27 | 6 | Missouri |
| Will Harris | 25 | CB | 6-1 | 206 | 26 | 4 | Boston College |
| T.J. Hockenson | 88 | TE | 6-5 | 244 | 25 | 4 | Iowa |
| JuJu Hughes | 33 | S | 5-11 | 191 | 24 | 3 | Fresno State |
| Mike Hughes | 23 | CB | 5-10 | 191 | 25 | 5 | UCF |
| Aidan Hutchinson | 97 | DL | 6-7 | 264 | 22 | R | Michigan |
| Jonah Jackson | 73 | G | 6-4 | 311 | 25 | 3 | Ohio State |
| Jermar Jefferson | 28 | RB | 5-10 | 212 | 22 | 2 | Oregon State |
| Kerby Joseph | 31 | S | 6-1 | 203 | 21 | R | Illinois |
| Tommy Kraemer | 78 | G | 6-6 | 316 | 24 | 2 | Notre Dame |
| Chase Lucas | 36 | CB | 5-11 | 184 | 25 | R | Arizona State |
| Alim McNeill | 54 | DL | 6-2 | 325 | 22 | 2 | N.C. State |
| Ifeatu Melifonwu | 26 | S | 6-3 | 210 | 23 | 2 | Syracuse |
| James Mitchell | 82 | TE | 6-4 | 249 | 23 | R | Virginia Tech |
| Matt Nelson | 67 | T | 6-7 | 318 | 26 | 3 | Iowa |
| Jeff Okudah | 1 | CB | 6-1 | 205 | 23 | 3 | Ohio State |
| Julian Okwara | 99 | LB | 6-4 | 239 | 24 | 3 | Notre Dame |
| Levi Onwuzurike | 91 | DL | 6-3 | 295 | 24 | 2 | Washington |
| Amani Oruwariye | 24 | CB | 6-2 | 202 | 26 | 4 | Penn State |
| Bobby Price | 27 | CB | 6-3 | 208 | 24 | 3 | Norfolk State |
| Frank Ragnow | 77 | C | 6-5 | 309 | 26 | 5 | Arkansas |
| Kalif Raymond | 11 | WR | 5-8 | 180 | 28 | 6 | Holy Cross |
| Craig Reynolds | 46 | RB | 5-11 | 216 | 26 | 1 | Kutztown |
| Josh Reynolds | 8 | WR | 6-3 | 194 | 27 | 6 | Texas A&M |
| Malcolm Rodriguez | 44 | LB | 5-11 | 230 | 23 | R | Oklahoma State |
| Austin Seibert | 19 | K | 5-9 | 200 | 25 | 4 | Oklahoma |
| Penei Sewell | 58 | T | 6-5 | 335 | 21 | 2 | Oregon |
| Amon-Ra St. Brown | 14 | WR | 6-0 | 202 | 22 | 2 | USC |
| Logan Stenberg | 71 | G | 6-6 | 312 | 25 | 3 | Kentucky |
| D’Andre Swift | 32 | RB | 5-9 | 208 | 23 | 3 | Georgia |
| Demetrius Taylor | 62 | DL | 6-1 | 295 | 23 | R | Appalachian State |
| Halapoulivaati Vaitai | 72 | G | 6-6 | 328 | 29 | 7 | Texas Christian |
| Tracy Walker III | 21 | S | 6-1 | 206 | 27 | 5 | Louisiana-Lafayette |
| Jamaal Williams | 30 | RB | 6-0 | 224 | 27 | 6 | BYU |
| Josh Woods | 51 | LB | 6-1 | 230 | 26 | 4 | Maryland |
| Brock Wright | 89 | TE | 6-5 | 255 | 23 | 2 | Notre Dame |
| Shane Zylstra | 84 | TE | 6-4 | 239 | 25 | 1 | Minnesota State |
| Jameson Williams | 9 | WR | 6-1 | 180 | 21 | R | Alabama |
| Jason Cabinda | 45 | FB | 6-1 | 238 | 26 | 4 | Penn State |
| Jerry Jacobs | 39 | CB | 5-11 | 203 | 24 | 2 | Arkansas |
| Romeo Okwara | 95 | DL | 6-4 | 255 | 27 | 7 | Notre Dame |
| Josh Paschal | 93 | DL | 6-3 | 274 | 22 | R | Kentucky |