| Player Name | Jersey No | Pos | HT | WT | Age | Exp | College |
| Montravius Adams | 57 | DT | 6-4 | 304 | 27 | 6 | Auburn |
| Tyson Alualu | 94 | DE | 6-3 | 304 | 35 | 13 | California |
| Calvin Austin III | 19 | WR | 5-9 | 162 | 23 | R | Memphis |
| Chris Boswell | 9 | K | 6-2 | 185 | 31 | 8 | Rice |
| Miles Boykin | 13 | WR | 6-4 | 220 | 25 | 4 | Notre Dame |
| Devin Bush | 55 | LB | 5-11 | 234 | 24 | 4 | Michigan |
| Chase Claypool | 11 | WR | 6-4 | 238 | 24 | 3 | Notre Dame |
| Mason Cole | 61 | OL | 6-5 | 298 | 26 | 5 | Michigan |
| James Daniels | 78 | OL | 6-4 | 327 | 24 | 5 | Iowa |
| Jesse Davis | 73 | OT | 6-6 | 309 | 30 | 6 | Idaho |
| Kevin Dotson | 69 | OL | 6-4 | 321 | 25 | 3 | Louisiana |
| Terrell Edmunds | 34 | S | 6-1 | 217 | 25 | 5 | Virginia Tech |
| Minkah Fitzpatrick | 39 | S | 6-1 | 207 | 25 | 5 | Alabama |
| Pat Freiermuth | 88 | TE | 6-5 | 258 | 23 | 2 | Penn State |
| Zach Gentry | 81 | TE | 6-8 | 265 | 25 | 4 | Michigan |
| Kendrick Green | 53 | C | 6-4 | 315 | 23 | 2 | Illinois |
| Najee Harris | 22 | RB | 6-1 | 232 | 24 | 2 | Alabama |
| Pressley Harvin III | 6 | P | 6-0 | 255 | 23 | 2 | Georgia Tech |
| J.C. Hassenauer | 60 | C | 6-2 | 295 | 26 | 3 | Alabama |
| Cameron Heyward | 97 | DT | 6-5 | 295 | 33 | 12 | Ohio State |
| Connor Heyward | 83 | TE/FB | 6-0 | 230 | 23 | R | Michigan State |
| Alex Highsmith | 56 | LB | 6-4 | 242 | 25 | 3 | Charlotte |
| Myles Jack | 51 | LB | 6-1 | 255 | 27 | 7 | UCLA |
| Diontae Johnson | 18 | WR | 5-10 | 183 | 26 | 4 | Toledo |
| Damontae Kazee | 24 | S | 5-11 | 174 | 29 | 6 | San Diego State |
| Miles Killebrew | 28 | S | 6-2 | 222 | 29 | 7 | Southern Utah State |
| Christian Kuntz | 46 | LS | 6-1 | 228 | 28 | 2 | Duquesne |
| DeMarvin Leal | 98 | DE | 6-4 | 290 | 22 | R | Texas A&M |
| Isaiahh Loudermilk | 92 | DE | 6-7 | 293 | 24 | 2 | Wisconsin |
| Arthur Maulet | 35 | CB | 5-10 | 190 | 29 | 6 | Memphis |
| Dan Moore Jr. | 65 | OT | 6-5 | 315 | 23 | 2 | Texas A&M |
| Tre Norwood | 21 | DB | 6-0 | 194 | 23 | 2 | Oklahoma |
| Larry Ogunjobi | 65 | DT | 6-3 | 305 | 28 | 6 | Charlotte |
| Chukwuma Okorafor | 76 | OL | 6-6 | 320 | 25 | 5 | Western Michigan |
| Gunner Olszewski | 89 | WR | 6-0 | 190 | 25 | 4 | Bemidji State |
| George Pickens | 14 | WR | 6-3 | 200 | 21 | R | Georgia |
| Kenny Pickett | 8 | QB | 6-3 | 220 | 24 | R | Pittsburgh |
| James Pierre | 42 | CB | 6-2 | 185 | 25 | 3 | Florida Atlantic |
| Malik Reed | 59 | LB | 6-2 | 235 | 26 | 4 | Nevada |
| Mark Robinson | 93 | LB | 5-11 | 235 | 23 | R | Mississippi |
| Mason Rudolph | 2 | QB | 6-5 | 235 | 27 | 5 | Oklahoma State |
| Steven Sims | 82 | WR | 5-10 | 176 | 25 | 3 | Kansas |
| Benny Snell Jr. | 24 | RB | 5-10 | 224 | 24 | 4 | Kentucky |
| Robert Spillane | 41 | LB | 6-1 | 229 | 26 | 4 | Western Michigan |
| Cameron Sutton | 20 | CB | 5-11 | 188 | 27 | 6 | Tennessee |
| Mitch Trubisky | 10 | QB | 6-2 | 220 | 28 | 6 | North Carolina |
| Derrek Tuszka | 48 | LB | 6-5 | 246 | 26 | 3 | North Dakota State |
| Levi Wallace | 29 | CB | 6-0 | 179 | 27 | 5 | Alabama |
| Jaylen Warren | 30 | RB | 5-8 | 215 | 23 | R | Oklahoma State |
| Derek Watt | 44 | FB | 6-2 | 234 | 29 | 7 | Wisconsin |
| T.J. Watt | 90 | LB | 6-4 | 252 | 27 | 6 | Wisconsin |
| Ahkello Witherspoon | 25 | CB | 6-2 | 195 | 27 | 6 | Colorado |
| Chris Wormley | 95 | DL | 6-5 | 300 | 29 | 6 | Michigan |
| Karl Joseph | 38 | S | 5-10 | 200 | 28 | 7 | West Virginia |
| Jeremy McNichols | 33 | RB | 5-9 | 214 | 26 | 3 | Boise State |
| Anthony Miller | 17 | WR | 5-11 | 199 | 27 | 5 | Memphis |
| Carlins Platel | 30 | DB | 6-1 | 205 | 23 | R | South Carolina |