| Player Name | Jersey No | Pos | HT | WT | Age | Exp | College |
| Calvin Anderson | 76 | T | 6-5 | 300 | 26 | 4 | Texas |
| Essang Bassey | 34 | CB | 5-10 | 190 | 23 | 3 | Wake Forest |
| Andrew Beck | 83 | FB/TE | 6-3 | 255 | 26 | 4 | Texas |
| Jacob Bobenmoyer | 46 | LS | 6-2 | 235 | 25 | 3 | Northern Colorado |
| Garett Bolles | 72 | T | 6-5 | 300 | 30 | 6 | Utah |
| Nik Bonitto | 42 | OLB | 6-3 | 240 | 22 | R | Oklahoma |
| Mike Boone | 26 | RB | 5-10 | 206 | 27 | 5 | Cincinnati |
| Baron Browning | 56 | LB | 6-3 | 240 | 23 | 2 | Ohio State |
| Bradley Chubb | 55 | OLB | 6-4 | 275 | 26 | 5 | North Carolina State |
| Tyrie Cleveland | 16 | WR | 6-2 | 205 | 24 | 3 | Florida |
| Jonathon Cooper | 53 | OLB | 6-4 | 257 | 24 | 2 | Ohio State |
| Lloyd Cushenberry III | 79 | C | 6-4 | 315 | 24 | 3 | LSU |
| Ronald Darby | 23 | CB | 5-11 | 193 | 28 | 8 | Florida State |
| Greg Dulcich | 80 | TE | 6-4 | 245 | 22 | R | UCLA |
| Cameron Fleming | 73 | T | 6-5 | 320 | 30 | 9 | Stanford |
| Graham Glasgow | 61 | G | 6-6 | 310 | 30 | 7 | Michigan |
| Melvin Gordon III | 25 | RB | 6-1 | 215 | 29 | 8 | Wisconsin |
| Randy Gregory | 5 | OLB | 6-5 | 242 | 29 | 5 | Nebraska |
| Jonas Griffith | 50 | ILB | 6-4 | 250 | 25 | 2 | Indiana State |
| KJ Hamler | 1 | WR | 5-9 | 178 | 23 | 3 | Penn State |
| Matt Henningsen | 91 | DE | 6-3 | 291 | 23 | R | Wisconsin |
| Kareem Jackson | 22 | S | 5-10 | 183 | 34 | 13 | Alabama |
| Jerry Jeudy | 10 | WR | 6-1 | 193 | 23 | 3 | Alabama |
| Josey Jewell | 47 | ILB | 6-2 | 236 | 27 | 5 | Iowa |
| D.J. Jones | 97 | DT | 6-0 | 305 | 27 | 6 | Mississippi |
| Dre’Mont Jones | 93 | DE | 6-3 | 281 | 25 | 4 | Ohio State |
| P.J. Locke | 37 | S | 5-10 | 202 | 25 | 3 | Texas |
| Damarri Mathis | 27 | CB | 5-11 | 195 | 23 | R | Pittsburgh |
| Brandon McManus | 8 | K | 6-3 | 201 | 31 | 9 | Temple |
| Quinn Meinerz | 77 | G/C | 6-3 | 320 | 23 | 2 | Wisconsin-Whitewater |
| Michael Ojemudia | 13 | CB | 6-1 | 200 | 24 | 3 | Iowa |
| Albert Okwuegbunam | 85 | TE | 6-5 | 258 | 24 | 3 | Missouri |
| Aaron Patrick | 94 | OLB | 6-4 | 245 | 29 | 2 | Eastern Kentucky |
| Dalton Risner | 66 | G | 6-5 | 312 | 27 | 4 | Kansas State |
| Brett Rypien | 4 | QB | 6-2 | 202 | 26 | 3 | Boise State |
| Eric Saubert | 82 | TE | 6-5 | 253 | 28 | 5 | Drake |
| Justin Simmons | 31 | S | 6-2 | 202 | 28 | 7 | Boston College |
| Alex Singleton | 49 | ILB | 6-2 | 240 | 28 | 4 | Montana State |
| Caden Sterns | 30 | S | 6-1 | 207 | 22 | 2 | Texas |
| Justin Strnad | 40 | ILB | 6-3 | 235 | 26 | 3 | Wake Forest |
| Pat Surtain II | 2 | CB | 6-2 | 202 | 22 | 2 | Alabama |
| Courtland Sutton | 14 | WR | 6-4 | 216 | 26 | 5 | Southern Methodist |
| Billy Turner | 57 | OL | 6-5 | 310 | 30 | 9 | North Dakota State |
| Delarrin Turner-Yell | 32 | S | 5-11 | 200 | 22 | R | Oklahoma |
| Eyioma Uwazurike | 96 | DL | 6-6 | 320 | 24 | R | Iowa State |
| Jalen Virgil | 17 | WR | 6-1 | 210 | 24 | R | Appalachian State |
| Corliss Waitman | 17 | P | 6-2 | 210 | 27 | 1 | South Alabama |
| Montrell Washington | 12 | WR | 5-10 | 170 | 23 | R | Samford |
| Luke Wattenberg | 60 | C | 6-5 | 300 | 24 | R | Washington |
| DeShawn Williams | 99 | DL | 6-1 | 291 | 29 | 4 | Clemson |
| Javonte Williams | 33 | RB | 5-10 | 220 | 22 | 2 | North Carolina |
| K’Waun Williams | 21 | CB | 5-9 | 185 | 31 | 8 | Pittsburgh |
| Russell Wilson | 3 | QB | 5-11 | 215 | 33 | 11 | Wisconsin |
| Christopher Allen | 45 | OLB | 6-4 | 242 | 24 | R | Alabama |
| Damarea Crockett | 28 | RB | 5-11 | 225 | 24 | 2 | Missouri |
| Brandon Johnson | 89 | WR | 6-2 | 195 | 24 | R | UCF |
| Tim Patrick | 81 | WR | 6-4 | 212 | 28 | 5 | Utah |
| Tom Compton | 69 | OL | 6-5 | 315 | 33 | 10 | South Dakota |