| Player Name | Jersey No | Pos | HT | WT | Age | Exp | College |
| Nick Allegretti | 73 | G | 6-4 | 310 | 26 | 4 | Illinois |
| Blake Bell | 81 | TE | 6-6 | 252 | 31 | 8 | Oklahoma |
| Nick Bolton | 32 | LB | 5-11 | 237 | 22 | 2 | Missouri |
| Orlando Brown | 57 | T | 6-8 | 340 | 26 | 5 | Oklahoma |
| Shane Buechele | 6 | QB | 6-0 | 210 | 24 | 2 | SMU |
| Michael Burton | 45 | FB | 5-11 | 242 | 30 | 8 | Rutgers |
| Deon Bush | 26 | DB | 6-0 | 200 | 29 | 7 | Miami |
| Harrison Butker | 7 | K | 6-4 | 199 | 27 | 6 | Georgia Tech |
| Leo Chenal | 54 | LB | 6-3 | 250 | 21 | R | Wisconsin |
| Geron Christian | 74 | T | 6-5 | 298 | 25 | 5 | Louisville |
| Frank Clark | 55 | DE | 6-3 | 272 | 29 | 8 | Michigan |
| Bryan Cook | 6 | S | 6-1 | 206 | 22 | R | Cincinnati |
| Mike Danna | 51 | DE | 6-2 | 257 | 24 | 3 | Michigan |
| Carlos Dunlap | 8 | DE/OLB | 6-6 | 277 | 33 | 13 | Florida |
| Clyde Edwards-Helaire | 25 | RB | 5-7 | 207 | 23 | 3 | LSU |
| Rashad Fenton | 27 | CB | 5-11 | 193 | 25 | 4 | South Carolina |
| Jody Fortson | 88 | TE | 6-4 | 226 | 26 | 2 | Valdosta State |
| Willie Gay | 50 | LB | 6-1 | 243 | 24 | 3 | Mississippi State |
| Noah Gray | 83 | TE | 6-3 | 240 | 23 | 2 | Duke |
| Mecole Hardman | 17 | WR | 5-10 | 187 | 24 | 4 | Georgia |
| Darius Harris | 47 | LB | 6-2 | 232 | 26 | 3 | Middle Tennessee |
| Chad Henne | 4 | QB | 6-3 | 230 | 37 | 15 | Michigan |
| Malik Herring | 94 | DE | 6-3 | 275 | 24 | 1 | Georgia |
| Creed Humphrey | 52 | C | 6-4 | 302 | 23 | 2 | Oklahoma |
| Chris Jones | 95 | DT | 6-6 | 310 | 28 | 7 | Mississippi State |
| Ronald Jones | 2 | RB | 5-11 | 205 | 25 | 5 | USC |
| Joshua Kaindoh | 59 | DE | 6-6 | 260 | 23 | 2 | Florida State |
| George Karlaftis | 56 | DE | 6-4 | 263 | 21 | R | Purdue |
| Travis Kelce | 87 | TE | 6-5 | 250 | 32 | 10 | Cincinnati |
| Darian Kinnard | 75 | T | 6-5 | 322 | 22 | R | Kentucky |
| Chris Lammons | 29 | CB | 5-9 | 194 | 26 | 3 | South Carolina |
| Patrick Mahomes | 15 | QB | 6-2 | 225 | 26 | 6 | Texas Tech |
| Trent McDuffie | 21 | CB | 5-11 | 193 | 21 | R | Washington |
| Jerick McKinnon | 1 | RB | 5-9 | 209 | 30 | 9 | Georgia Southern |
| Skyy Moore | 24 | WR | 5-10 | 195 | 21 | R | Western Michigan |
| Derrick Nnadi | 91 | DT | 6-1 | 317 | 26 | 5 | Florida State |
| Isiah Pacheco | 10 | RB | 5-10 | 216 | 23 | R | Rutgers |
| Justin Reid | 20 | S | 6-1 | 207 | 25 | 5 | Stanford |
| Khalen Saunders | 99 | DT | 6-0 | 324 | 26 | 4 | Western Illinois |
| Trey Smith | 65 | OL | 6-6 | 321 | 23 | 2 | Tennessee |
| JuJu Smith-Schuster | 9 | WR | 6-1 | 215 | 25 | 6 | USC |
| L’Jarius Sneed | 38 | CB | 6-1 | 192 | 25 | 3 | Louisiana Tech |
| Juan Thornhill | 22 | S | 6-0 | 205 | 26 | 4 | Virginia |
| Joe Thuney | 62 | OL | 6-5 | 304 | 29 | 7 | N.C. State |
| Tommy Townsend | 5 | P | 6-1 | 191 | 25 | 3 | Florida |
| Marquez Valdes-Scantling | 11 | WR | 6-4 | 206 | 27 | 5 | University of South Florida |
| Prince Tega Wanogho | 70 | T | 6-5 | 308 | 24 | 2 | Auburn |
| Jaylen Watson | 35 | CB | 6-2 | 197 | 23 | R | Washington State |
| Justin Watson | 84 | WR | 6-2 | 215 | 26 | 5 | Pennsylvania |
| Tershawn Wharton | 98 | DT | 6-1 | 280 | 24 | 3 | Missouri S&T |
| Joshua Williams | 23 | DB | 6-3 | 193 | 22 | R | Fayetteville State University |
| James Winchester | 41 | LS | 6-3 | 209 | 33 | 8 | Oklahoma |
| Andrew Wylie | 77 | G | 6-5 | 304 | 28 | 5 | Eastern Michigan |
| Justyn Ross | 8 | WR | 6-4 | 210 | 22 | R | Clemson |
| Lucas Niang | 67 | OL | 6-6 | 315 | 24 | 2 | TCU |